Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Targeting secreted cytokine BMP9 gates the attenuation of hepatic fibrosis.

doi: 10.1016/j.bbadis.2017.12.008

Figure Lengend Snippet: Figure 1 BMP9 is overexpressed in human patients

Article Snippet: ELISA An in-house-produced anti-BMP9 mAb recognizing both mouse and human BMP9 was used as the capture antibody, and a biotin-linked anti-Hyaluronic acid(R&D Systems, Abingdon ) and anti-BMP9 antibody (R&D Systems, Abingdon, UK; Abcam, Cambridge, USA) was used as the detection antibody.

Techniques:

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Targeting secreted cytokine BMP9 gates the attenuation of hepatic fibrosis.

doi: 10.1016/j.bbadis.2017.12.008

Figure Lengend Snippet: Figure 3 Adenovirus-mediated Bmp9 knockdown attenuates liver fibrosis

Article Snippet: ELISA An in-house-produced anti-BMP9 mAb recognizing both mouse and human BMP9 was used as the capture antibody, and a biotin-linked anti-Hyaluronic acid(R&D Systems, Abingdon ) and anti-BMP9 antibody (R&D Systems, Abingdon, UK; Abcam, Cambridge, USA) was used as the detection antibody.

Techniques: Knockdown

Journal: iScience

Article Title: Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice

doi: 10.1016/j.isci.2021.102488

Figure Lengend Snippet: Bivalent FSTL3-Fc is a potent inhibitor that specifically binds to activin A, GDF8, and GDF11 (A) Schematic presentation of the three isoforms of FST and FSTL3. NTD, N-terminal domain; FSD, follistatin domain; C, C-terminal domain; HBS, heparin-binding site. (B) Schematic presentation of bivalent FSTL3-Fc protein used in this study. See also for cDNA sequence. (C–G) In vitro binding assays between bi-FSTL3-Fc protein immobilized by anti-human Fc antibody and biotinylated ligands. The data represent mean ± SD from n = 3 independent experiments. Ligand-binding parameters for bi-FSTL3-Fc as determined by in vitro binding assays (G). ALK1-Fc is a positive control for BMP9.

Article Snippet: Since anti-BMP9 (MAB3209; R&D) recognizes non-reduced BMP9 dimer, SDS-PAGE was performed under non-reduced conditions.

Techniques: Binding Assay, Sequencing, In Vitro, Ligand Binding Assay, Positive Control

Journal: iScience

Article Title: Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice

doi: 10.1016/j.isci.2021.102488

Figure Lengend Snippet:

Article Snippet: Since anti-BMP9 (MAB3209; R&D) recognizes non-reduced BMP9 dimer, SDS-PAGE was performed under non-reduced conditions.

Techniques: Recombinant, Antibody Purification, Expressing, Labeling, Quantitation Assay, Enzyme-linked Immunosorbent Assay, Reporter Assay, Software, Cell Counting, Microscopy